Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2.

 

產(chǎn)品性質(zhì)

別名	MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein k
SwissProt ID	Q02750/P36507
基因ID	5604/5605
免疫原	Recombinant protein of human MEK1/2.
緩沖液	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.
分子量	Calculated MW: 43,44 kDa; Observed MW: 43,44 kDa.
克隆類型	Monoclonal Antibody
亞型	IgG
宿主	Rabbit
物種反應(yīng)	Human, Mouse, Rat
推薦應(yīng)用	WB, IHC-F, IHC-P, ICC/IF,IP
純化方式	Affinity Purified

 

應(yīng)用

稀釋比 WB:1/500-1/1000; IHC:1/50-1/100; IF: 1/50-1/200; IP:1/20

 

儲存條件

-25~-15℃保存

 

注意事項(xiàng)

1. 避免反復(fù)凍融。

2. 本產(chǎn)品僅作科研用途。

3. 為了您的安全和健康，請穿實(shí)驗(yàn)服并佩戴一次性手套操作。

 

產(chǎn)品數(shù)據(jù)

Western blot

Western blot analysis of MEK1/2 in K562, C6, 3T3, HeLa lysates using MEK1/2 antibody.

 

Immunocytochemistry

Immunocytochemistry analysis of MEK1/2(green) in HeLa using MEK1/2 antibody, and DAPI(blue).

 

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using MEK1/2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

 

Ver.CN20231220