The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.

 

產(chǎn)品性質(zhì)

別名	E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
SwissProt ID	Q01094
基因ID	1869
免疫原	A synthetic peptide of human E2F1.
緩沖液	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.
分子量	Calculated MW: 47 kDa; Observed MW: 70 kDa.
克隆類型	Monoclonal Antibody
亞型	IgG
宿主	Rabbit
物種反應(yīng)	Human, Mouse, Rat
推薦應(yīng)用	WB, IHC-F, IHC-P, ICC/IF, IP
純化方式	Affinity Purified

 

應(yīng)用

稀釋比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200; IP:1/20

 

儲存條件

-25~-15℃保存

 

注意事項

1. 避免反復(fù)凍融。

2. 本產(chǎn)品僅作科研用途。

3. 為了您的安全和健康，請穿實驗服并佩戴一次性手套操作。

 

產(chǎn)品數(shù)據(jù)

Western blot

Western blot analysis of E2F1 in MOLT4 lysates using E2F1 antibody.

Western blot analysis of E2F1 in C6, 3T3 lysates using E2F1 antibody.

 

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human tonsil using E2F1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

 

Immunocytochemistry

Immunocytochemistry analysis of E2F1(green) in HeLa using E2F1 antibody, and DAPI(blue).

 

Ver.CN20231220