導(dǎo)讀
傳統(tǒng)酶切克隆
無(wú)縫克隆
1. 不受片段、載體序列的限制，可任意組裝
2. 可同時(shí)無(wú)縫地拼接多個(gè)片段
3. 操作簡(jiǎn)便，時(shí)間上大大節(jié)省
1. 線性化載體制備
2. PCR獲取目的插入片段
3. 重組反應(yīng)
1. Jiang, Y., et al., Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic fungi[J]. Science, 2017. (IF 37.205)
2. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019. (IF 31.398)
3. Xing, Y.H., et al., SLERT Regulates DDX21 Rings Associated with Pol I Transcription[J]. Cell, 2017. 169(4): p. 664-678.e16. (IF 31.398)
4. Wang Y, Hu SB, et al. Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria[J]. Nat Cell Biol. 2018 Oct;20(10):1145-1158. (IF 20.46)
5. Li X., et al., Coordinated circRNA Biogenesis and Function with NF90/NF110 in Viral Infection[J]. Mol Cell. 2017 Jul 20;67(2):214-227.e7. (IF 14.548)
6. Yao R W, Xu G, Wang Y, et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus[J]. Molecular cell, 2019. (IF 14.548)
7. Wu P, Zhang T, Liu B, et al. Mechano-regulation of peptide-MHC class I conformations determines TCR antigen recognition[J]. Molecular cell, 2019, 73(5): 1015-1027. e7. (IF 14.548)
8. Qiao HH, Wang F, et al. An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila[J]. Nat Commun. 2018 Oct 8;9(1):4160. (IF 12.353)
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