Recombinant SARS-COV-2 Spike RBD (N501Y, K417N,E484K)(His Tag)
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產(chǎn)品說明書
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SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS?CoV and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 .The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). Before binding to the ACE-2 receptor, structural analysis of the S1 trimer shows that only one of the three RBD domains is in the "up" conformation. This is an unstable and transient state that passes between trimeric subunits but is nevertheless an exposed state to be targeted for neutralizing antibody therapy. Polyclonal antibodies to the RBD of the SARS-CoV-2 protein have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy.
產(chǎn)品性質(zhì)
|
別名 |
S protein RBD; S glycoprotein RBD; Spike protein RBD |
|
Accession |
QHD43416.1 |
|
表達(dá)區(qū)間及表達(dá)系統(tǒng) |
Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Arg319 - Phe541 ( N501Y, K417N, E484K). |
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分子量 |
Approximately 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. |
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純度 |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性 |
ELISA Data：Immobilized SARS-COV-2 Spike RBD (N501Y, K417N, E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 23.3ng/ml determined by ELISA. |
|
內(nèi)毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制劑 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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復(fù)溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃至-80℃保存，一年有效期。
復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時(shí)分裝凍存，避免反復(fù)凍融。
注意事項(xiàng)
1. 避免反復(fù)凍融。
2. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
3. 本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
HB220811
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