產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻
Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2).
產(chǎn)品性質(zhì)
|
別名 |
S protein RBD; S glycoprotein RBD; Spike protein RBD |
|
Accession |
QHD43416.1 |
|
表達區(qū)間及表達系統(tǒng) |
Recombinant SARS-CoV-2 Spike RBD (Omicron BA.4/BA.5/BA.5.1.3/BA.5.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H). |
|
分子量 |
Approximately 26.05 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
|
純度 |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性 |
ELISA Data：Immobilized SARS-CoV-2 Spike RBD (Omicron BA.4/BA.5/BA.5.1.3/BA.5.2) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.7ng/ml determined by ELISA. |
|
內(nèi)毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制劑 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
|
復(fù)溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運輸與保存方法
冰袋運輸。-20 ℃保存，一年有效期。
復(fù)溶后，-20 °C，在未開封狀態(tài)下保存 3-6 個月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時分裝凍存，避免反復(fù)凍融。
注意事項
- 避免反復(fù)凍融。
- 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
- 本產(chǎn)品僅作科研用途！
HB221222
推薦商品
