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      Recombinant SARS-COV-2 Spike RBD(Omicron XBB) Protein,  His Tag
       
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      產(chǎn)品說明書
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻(xiàn)
                  
       
       
      


      Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2).
      


       
      


      產(chǎn)品信息
      


      
	
		
      
			
			
      別名
      
			      		
			
			
      S protein RBD; S glycoprotein RBD; Spike protein RBD
      
			      		
		
      
		
      
			
			
      Accession
      
			      		
			
			
      QHD43416.1
      
			      		
		
      
		
      
			
			
      表達(dá)區(qū)間及表達(dá)系統(tǒng)
      
			      		
			
			
      Recombinant SARS-CoV-2 Spike RBD (Omicron XBB) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H).
      
			      		
		
      
		
      
			
			
      分子量
      
			      		
			
			
      Approximately 26.21 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
      
			      		
		
      
		
      
			
			
      純度
      
			      		
			
			
      > 95% as determined by SDS-PAGE and HPLC.
      
			      		
		
      
		
      
			
			
      活性
      
			      		
			
			
      ELISA Data：Immobilized SARS-CoV-2 Spike RBD (Omicron XBB) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
      
			      		
		
      
		
      
			
			
      內(nèi)毒素
      
			      		
			
			
      < 1.0 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      制劑
      
			      		
			
			
      Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). 
      
			      		
		
      
		
      
			
			
      復(fù)溶
      
			      		
			
			
      Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
      
			      		
		
      
	      

      


       
      


      儲存條件
      


      The product should be stored at -25~-15℃.
      


      1 month, 2 ~8 °C under sterile conditions after reconstitution.
      


      3 months, -25~-15℃ °C under sterile conditions after reconstitution.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      注意事項
      


      1.Please operate with lab coats and disposable gloves，for your safety。
      


      2.This product is for research use only. 
      


       
      

         
       
       
       
       
       
       
       
      400-6111-883