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Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). First detected in India in December 2020, the B.1.617.2, or Delta variant, is considered a Variant of Concern (VOC) as it contains several mutations in the RBD domain that potentially affect viral fitness and transmissibility: L452R and T478K.
產(chǎn)品性質(zhì)
別名 |
S protein RBD; S glycoprotein RBD; Spike protein RBD |
Accession |
QHD43416.1 |
表達(dá)區(qū)間及表達(dá)系統(tǒng) |
Recombinant SARS-COV-2 Spike RBD (Delta B.1.617.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(L452R, T478K). |
分子量 |
Approximately 26.26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
純度 |
> 95% as determined by SDS-PAGE and HPLC. |
活性 |
ELISA Data:Immobilized SARS-CoV-2 Spike RBD (Delta B.1.617.2) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. |
內(nèi)毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
制劑 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
復(fù)溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20 ℃保存,一年有效期。
復(fù)溶后,-20 °C,在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后,2-8°C 保存 2-7 天。
建議第一次使用時(shí)分裝凍存,避免反復(fù)凍融。
注意事項(xiàng)
1.避免反復(fù)凍融。
2.為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
3.本產(chǎn)品僅作科研用途!
HB221112
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