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      Recombinant SARS-COV-2 Spike RBD(Delta B.1.617.2) Protein, His Tag

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      產(chǎn)品說明書

      FAQ

      COA

      已發(fā)表文獻(xiàn)

       

      Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). First detected in India in December 2020, the B.1.617.2, or Delta variant, is considered a Variant of Concern (VOC) as it contains several mutations in the RBD domain that potentially affect viral fitness and transmissibility: L452R and T478K.   

       

      產(chǎn)品性質(zhì)

      別名

      S protein RBD; S glycoprotein RBD; Spike protein RBD

      Accession

      QHD43416.1

      表達(dá)區(qū)間及表達(dá)系統(tǒng)

      Recombinant SARS-COV-2 Spike RBD (Delta B.1.617.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(L452R, T478K). 

      分子量

      Approximately 26.26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

      純度

      > 95% as determined by SDS-PAGE and HPLC.

      活性

      ELISA DataImmobilized SARS-CoV-2 Spike RBD (Delta B.1.617.2) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.

      內(nèi)毒素

      1.0 EU per 1μg of the protein by the LAL method.

      制劑

      Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

      復(fù)溶

      Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

       

      運(yùn)輸與保存方法

      冰袋運(yùn)輸。-20 保存,一年有效期。

      復(fù)溶后,-20 °C,在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后,2-8°C 保存 2-7 天。

      建議第一次使用時(shí)分裝凍存,避免反復(fù)凍融。

       

      注意事項(xiàng)

      1.避免反復(fù)凍融。

      2.為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

      3.本產(chǎn)品僅作科研用途!

      HB221112

      400-6111-883