SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses. The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). Several emerging SARS-CoV-2 genomes have been identified including the Omicron, or B.1.1.529, variant. First identified in November 2021 in South Africa, the Omicron variant quickly became the predominant SARS-CoV-2 variant and is considered a variant of concern (VOC). The Omicron variant contains 15 mutations in RBD domain that potentially affect viral fitness and transmissibility. The majority of the mutations are involved in ACE-2 binding and Omicron binds ACE-2 with greater affinity, potentially explaining its increased transmissibility. Several of these mutations are also identified in facilitating immune escape and reducing neutralization activity to several monoclonal antibodies.

 

產(chǎn)品性質(zhì)

別名	S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession	QHO60594.1
表達(dá)區(qū)間及表達(dá)系統(tǒng)	Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H).
分子量	Approximately 76.38 kDa. Due to glycosylation, the protein migrates to 130-145 kDa based on Tris-Bis PAGE result.
純度	> 95% as determined by SDS-PAGE and HPLC.
活性	ELISA Data：Immobilized Human ACE2, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag with the EC50 of 0.8μg/ml determined by ELISA.
內(nèi)毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制劑	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
復(fù)溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

運輸與保存方法

 

冰袋運輸。-20℃至-80℃保存，一年有效期。

復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復(fù)溶后，2-8°C 保存 2-7 天。

建議第一次使用時分裝凍存，避免反復(fù)凍融。

 

注意事項

1. 避免反復(fù)凍融。

2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。

3. 本產(chǎn)品僅作科研用途！

 

產(chǎn)品數(shù)據(jù)

 

HB220811