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      Recombinant SARS-COV-2 Spike S1(Delta B.1.617.2) Protein,   His Tag
       
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      產(chǎn)品說明書
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻
                  
       
       
      


      Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). First detected in India in December 2020, the B.1.617.2, or Delta variant, is considered a Variant of Concern (VOC) .
      


       
      


      產(chǎn)品信息
      


      
	
		
      
			
			
      別名
      
			      		
			
			
      S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
      
			      		
		
      
		
      
			
			
      Accession
      
			      		
			
			
      QHD43416.1
      
			      		
		
      
		
      
			
			
      表達區(qū)間及表達系統(tǒng)
      
			      		
			
			
      Recombinant SARS-CoV-2 Spike S1 (Delta B.1.617.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln14-Arg683(T19R, G142D, E156G, HR157-158 del, L452R, T478K, D614G, P681R). 
      
			      		
		
      
		
      
			
			
      分子量
      
			      		
			
			
      Approximately 75.92 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
      
			      		
		
      
		
      
			
			
      純度
      
			      		
			
			
      > 95% as determined by Tris-Bis PAGE and HPLC.
      
			      		
		
      
		
      
			
			
      活性
      
			      		
			
			
      ELISA Data：Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) , His Tag at 0.5μg/ml (100 μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA.
      
			      		
		
      
		
      
			
			
      內(nèi)毒素
      
			      		
			
			
      < 1.0 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      制劑
      
			      		
			
			
      Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
      
			      		
		
      
		
      
			
			
      復(fù)溶
      
			      		
			
			
      Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
      
			      		
		
      
	      

      


       
      


      儲存條件
      


      The product should be stored at -25~-15℃ for 1 year.
      


      1 month, 2 ~8 °C under sterile conditions after reconstitution.
      


      3 months, -25~-15℃ °C under sterile conditions after reconstitution.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      注意事項
      


      1.Please operate with lab coats and disposable gloves，for your safety。
      


      2.This product is for research use only. 
      

         
       
       
       
       
       
       
       
      400-6111-883