產(chǎn)品說明書
FAQ
COA
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產(chǎn)品描述
DAPI Fluoromount-G™是一種含有藍色細胞核熒光探針-DAPI（Em=455 nm）的水溶性封片劑，用來對最后一步染色是在水溶液體系中完成的玻片樣本進行封片，可以顯著減緩各種常見熒光染料的熒光淬滅現(xiàn)象。本封片劑具有半永久性封固性，可用作長期保存玻片樣本的制作。適用于細胞制備物和組織切片的封片，兼容于免疫熒光，免疫細胞化學，免疫組化等實驗。
本品已加DAPI，可同時完成核染和封片，使得實驗操作更簡便。封片劑干燥后形成一層牢固的涂層，光學透明，便于后續(xù)熒光觀察和拍照。
運輸和保存方法
室溫運輸。室溫避光保存，一年有效。
使用方法
1） 根據(jù)實驗室已有的體系制備細胞離心涂片；
2） 固定和再水化細胞涂片；
3） 從染色最后一步的水溶性緩沖液中取出一片玻片，直接滴加一滴 DAPI Fluoromount-G™至細胞涂片，蓋上蓋玻片，用無紡紗布輕輕按壓蓋玻片以去除多余分封片劑和封固蓋玻片。
4） 室溫下避光靜置5 min，使得封片劑晾干后再進行普通熒光顯微鏡或者共聚焦顯微檢測。5） 若玻片樣本需長久保存，可使用指甲油或者中性樹膠（貨號：36313ES60）對蓋玻片四周進行密封。
注意事項
1）本品含有0.1%的疊氮鈉作為防腐劑，吞食和吸入有毒，操作時請做好防護。
2）為了您的安全和健康，請穿實驗服并戴一次性手套操作。
3）本產(chǎn)品僅作科研用途！
Q：該產(chǎn)品需要低溫貯藏嗎？
A：室溫避光保存即可。
Q：使用該產(chǎn)品后多久可進行顯微檢測？
A：一般室溫下避光靜置 5 min，使得封片劑晾干后，再進行普通熒光顯微鏡或者共聚焦顯微檢測。
Q：該產(chǎn)品可使玻片長久保存嗎？
A：不可。若玻片樣本需長久保存，可使用指甲油或者中性樹膠對蓋玻片四周進行密封。
Q：該產(chǎn)品可用于免疫熒光、免疫細胞化學、免疫組化實驗嗎？
A：可以。
Q：該產(chǎn)品可用于活細胞嗎？
A：不推薦，對于染色活細胞膜染料和線粒體染料的抗淬滅效果不佳，建議抗熒光淬滅封片劑用于固定透化的細胞或者組織。
Q：封片后片子一般如何保存？
A：4度保存。
Q：封片劑可以讓熒光保持多久呢? DAPI一般可以保存多久？
A：取決于染料的本身，有的染料本身就特別容易淬滅，也是維持不了很久的，2-3天左右;對于那種相對穩(wěn)定的染料，可以維持1周以上；DAPI比較穩(wěn)定，可保存一周左右。
Q：此產(chǎn)品中DAPI試劑的濃度是多少？
A：1.5 ug/mL.
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