產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻(xiàn)
SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses. The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). Several emerging SARS-CoV-2 genomes have been identified including the Omicron, or B.1.1.529, variant. First identified in November 2021 in South Africa, the Omicron variant quickly became the predominant SARS-CoV-2 variant and is considered a variant of concern (VOC). The Omicron variant contains 15 mutations in RBD domain that potentially affect viral fitness and transmissibility. The majority of the mutations are involved in ACE-2 binding and Omicron binds ACE-2 with greater affinity, potentially explaining its increased transmissibility.
產(chǎn)品性質(zhì)
|
別名 |
S protein; Spike glycoprotein; S glycoprotein; COVID-19 |
|
Accession |
QHO60594.1 |
|
表達(dá)區(qū)間及表達(dá)系統(tǒng) |
Recombinant SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. |
|
分子量 |
Approximately 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Tris-Bis PAGE result. |
|
純度 |
> 95% as determined by SDS-PAGE. |
|
活性 |
ELISA Data：Immobilized SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. |
|
內(nèi)毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制劑 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
復(fù)溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運輸與保存方法
冰袋運輸。-20℃至-80℃保存，一年有效期。
復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時分裝凍存，避免反復(fù)凍融。
注意事項
1. 避免反復(fù)凍融。
2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
3. 本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
HB220811
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