細(xì)胞轉(zhuǎn)染實(shí)驗(yàn)從開始設(shè)計(jì)到最終的效果檢測，可以由6個過程組成：核酸準(zhǔn)備、試劑選擇、細(xì)胞鋪板、轉(zhuǎn)染操作、細(xì)胞培養(yǎng)、效果檢測。每一步都會影響最終的結(jié)果，均存在不同的注意事項(xiàng)。上個月小翌分享了前3個過程的注意事項(xiàng)：
細(xì)胞轉(zhuǎn)染注意事項(xiàng)，你都get了嗎？（上）
本期分享后面3個過程的注意事項(xiàng)：
轉(zhuǎn)染操作
1.條件摸索與優(yōu)化：
2.無血清配置復(fù)合物：
3.孵育時間：
4.抗生素影響：
1.優(yōu)良的培養(yǎng)試劑：
2.是否換液：
1.檢測方法的選擇：
2.檢測時間的選擇：
3.穩(wěn)轉(zhuǎn)篩選：
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應(yīng)用場景 | 名稱 | 貨號 | 規(guī)格 | 價格（元） |
細(xì)胞類型：貼壁/懸浮 | Hieff TransTM Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑 | 40802ES01 | 100 μL | 168.00 |
40802ES02 | 0.5 mL | 738.00 | ||
40802ES03 | 1.0 mL | 1308.00 | ||
40802ES08 | 5×1mL | 4878.00 | ||
細(xì)胞類型：貼壁/懸浮 核酸類型：DNA | Calcium Phosphate Cell Transfection Kit 磷酸鈣法細(xì)胞轉(zhuǎn)染試劑 | 40803ES70 | 200 T | 625.00 |
用途：病毒感染+DNA轉(zhuǎn)染 | 40804ES76 | 500 μL | 180.00 | |
40804ES86 | 5×500 μL | 500.00 | ||
細(xì)胞類型：懸浮 | Hieff TransTM Suspension Cell-Free Liposomal Transfection Reagent 懸浮細(xì)胞專用脂質(zhì)體核酸轉(zhuǎn)染試劑 | 40805ES01 | 100 μL | 228.00 |
40805ES02 | 0.5 mL | 948.00 | ||
40805ES03 | 1.0 mL | 1678.00 | ||
40805ES08 | 5×1 mL | 5268.00 | ||
細(xì)胞類型：貼壁/懸浮 核酸類型：siRNA、miRNA | Hieff TransTM in vitro siRNA/miRNA Transfection Reagent siRNA/miRNA體外轉(zhuǎn)染試劑 | 40806ES01 | 0.1 mL | 372.00 |
40806ES02 | 0.5 mL | 1472.00 | ||
40806ES03 | 1.0 mL | 2572.00 | ||
細(xì)胞類型：貼壁/懸浮 核酸類型：DNA、siRNA、miRNA | Hieff TransTM Universal Transfection Reagent Hieff TransTM通用型轉(zhuǎn)染試劑 | 40808ES02 | 0.5 mL | 1165.00 |
40808ES03 | 1 mL | 2065.00 | ||
40808ES08 | 5×1 mL | 7985.00 | ||
細(xì)胞類型：貼壁/懸浮 核酸類型：DNA | Polyethylenimine Linear(PEI) MW25000 線性PEI轉(zhuǎn)染試劑MW25000 | 40815ES03 | 1 g | 1855.00 |
40815ES08 | 5×1 g | 7255.00 | ||
細(xì)胞類型：貼壁/懸浮 核酸類型：DNA | Polyethylenimine Linear(PEI) MW40000（rapid lysis）線性PEI轉(zhuǎn)染試劑（速溶型）MW40000 | 40816ES02 | 100 mg | 655.00 |
40816ES03 | 1 g | 1855.00 |
