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      首頁(yè) / 細(xì)胞轉(zhuǎn)染 / 產(chǎn)品應(yīng)用詳情

      PEI轉(zhuǎn)染試劑與脂質(zhì)體轉(zhuǎn)染試劑選擇指南

      產(chǎn)品概述

      PEI轉(zhuǎn)染試劑與脂質(zhì)體轉(zhuǎn)染試劑是目前使用最廣泛的化學(xué)類(lèi)細(xì)胞轉(zhuǎn)染試劑,在基因功能研究、基因表達(dá)調(diào)控、突變分析,以及基因治療、細(xì)胞治療、蛋白生產(chǎn)、疫苗生產(chǎn)等方面應(yīng)用廣泛。

      針對(duì)DNA轉(zhuǎn)染試劑與RNA轉(zhuǎn)染試劑,翌圣均擁有雄厚的研發(fā)與生產(chǎn)團(tuán)隊(duì),不斷優(yōu)化配方,改良生產(chǎn)工藝,推出了多款以陽(yáng)離子脂質(zhì)體與陽(yáng)離子聚合物為基礎(chǔ)的產(chǎn)品,為廣大科研院校與企業(yè)提供全方位的產(chǎn)品,產(chǎn)品線覆蓋轉(zhuǎn)染試劑涉及的各個(gè)領(lǐng)域。

       

      產(chǎn)品優(yōu)勢(shì)

       

      高效性:適合瞬時(shí)轉(zhuǎn)染或者穩(wěn)定轉(zhuǎn)染細(xì)胞系。

      低毒性:轉(zhuǎn)染的細(xì)胞仍保持很好的活性。

      適應(yīng)性廣:普通細(xì)胞、難轉(zhuǎn)染的原代細(xì)胞全面覆蓋。

      操作簡(jiǎn)便:適合血清存在的培養(yǎng)基,轉(zhuǎn)染前后無(wú)需更換培養(yǎng)基。

      性價(jià)比高:經(jīng)濟(jì)實(shí)用,轉(zhuǎn)染效率高,價(jià)格低。

       

      選擇指南

       

      轉(zhuǎn)染試劑的選擇,需要根據(jù)實(shí)驗(yàn)?zāi)康呐c實(shí)驗(yàn)內(nèi)容,如轉(zhuǎn)染的物質(zhì)、具體的細(xì)胞、操作的便捷性等因素綜合考量,小編進(jìn)行了以下的區(qū)分,供大家參考。

      1、DNA/質(zhì)粒轉(zhuǎn)染試劑

      A10 kb以下DNA/質(zhì)粒

      產(chǎn)品名稱(chēng)

      核酸類(lèi)型

      細(xì)胞類(lèi)型

      優(yōu)點(diǎn)

      貨號(hào)

      規(guī)格

      Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑

      DNA(小于10 kb

      貼壁細(xì)胞

      1.轉(zhuǎn)染效率高
      2.用量少

      40802ES02/03/08

      0.5 mL/1 mL/5×1 mL

      磷酸鈣法細(xì)胞轉(zhuǎn)染試劑

      DNA(小于10 kb

      貼壁細(xì)胞

      1.毒性低
      2.操作簡(jiǎn)單
      3.用量少

      40803ES70

      200 T

      懸浮細(xì)胞專(zhuān)用脂質(zhì)體核酸轉(zhuǎn)染試劑

      DNA(小于10 kb

      懸浮細(xì)胞

      1.專(zhuān)門(mén)轉(zhuǎn)染懸浮細(xì)胞
      2.毒性低
      3.轉(zhuǎn)染效率高

      40805ES02/03/08

      0.5 mL/1 mL/5×1 mL

      Hieff Trans®通用型轉(zhuǎn)染試劑

      DNA(小于10 kb

      貼壁和懸浮細(xì)胞

      1.毒性低
      2.用量少

      40808ES02/03/08

      0.5 mL/1 mL/5×1 mL

       

      Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑

       

      Hieff Trans®脂質(zhì)體核酸轉(zhuǎn)染試劑是一種多用途的脂質(zhì)體轉(zhuǎn)染試劑,適用于DNA、RNA和寡核苷酸的轉(zhuǎn)染,對(duì)大多數(shù)真核細(xì)胞具有很高的轉(zhuǎn)染效率。其獨(dú)特的配方使其可直接加入培養(yǎng)基中,血清的存在不會(huì)影響轉(zhuǎn)染效率,這樣可以減少去除血清對(duì)細(xì)胞的損傷。轉(zhuǎn)染后不需要除去核酸-脂質(zhì)體復(fù)合物或更換新鮮培養(yǎng)基,也可在46小時(shí)后除去。

       

      1. Hieff Trans® 轉(zhuǎn)染試劑操作流程示意圖

      本產(chǎn)品以無(wú)菌的液體形式提供,通常情況下對(duì)于24孔板轉(zhuǎn)染,每次用1.5 μL左右,則1 mL約可做660次轉(zhuǎn)染;對(duì)于6孔板,每次用4 μL左右,則1 mL 約可做250次轉(zhuǎn)染;

       

      B、10 kb以上DNA/質(zhì)粒

      產(chǎn)品名稱(chēng)

      核酸類(lèi)型

      細(xì)胞類(lèi)型

      優(yōu)點(diǎn)

      貨號(hào)

      規(guī)格

      線性PEI轉(zhuǎn)染試劑MW25000

      DNA(大于10 kb的也可以轉(zhuǎn)染)

      貼壁細(xì)胞(較好)
      懸浮細(xì)胞(一般)

      1.轉(zhuǎn)染效率高
      2.用量少

      40815ES03/08

      1 g/5×1 g

      線性PEI轉(zhuǎn)染試劑MW40000(速溶型)

      DNA(大于10 kb的也可以轉(zhuǎn)染)

      貼壁細(xì)胞(較好)
      懸浮細(xì)胞(一般)

      1.轉(zhuǎn)染效率高
      2..用量少

      40816ES02/03

      100 mg/1 g

       

      Polyethylenimine Linear (PEI) MW40000rapid lysis) 線性PEI轉(zhuǎn)染試劑(速溶型)MW40000

      PEI 40000是一種分子量為40000的高電荷陽(yáng)離子聚合物,非常容易結(jié)合帶負(fù)電荷的核酸分子,形成復(fù)合物,并使該復(fù)合物進(jìn)入細(xì)胞中。PEI 40000是一種瞬時(shí)轉(zhuǎn)染試劑,細(xì)胞毒性低,轉(zhuǎn)染效率高,在HEK293CHO等細(xì)胞中基因表達(dá)效率較高。目前已經(jīng)驗(yàn)證線性PEI轉(zhuǎn)染試劑廣泛適用于多種細(xì)胞系包括HEK-293HEK293T、CHO-K1COS-1、COS-7NIH/3T3 、Sf9HepG2Hela細(xì)胞等。轉(zhuǎn)染效率高達(dá)80%~90%

       

      2、RNA轉(zhuǎn)染試劑

      產(chǎn)品名稱(chēng)

      核酸類(lèi)型

      細(xì)胞類(lèi)型

      優(yōu)點(diǎn)

      貨號(hào)

      規(guī)格

      Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑

      siRNA

      貼壁細(xì)胞

      1.轉(zhuǎn)染效率高
      2.用量少

      40802ES02/03/08

      0.5 mL/1 mL/5×1 mL

      Hieff Trans® siRNA/miRNA體外轉(zhuǎn)染試劑

      siRNA、miRNApre-miRNA, mimic miRNA, antimiRNA

      貼壁和懸浮細(xì)胞

      1.極好的基因沉默效率
      2.siRNA濃度低
      3.細(xì)胞毒性低

      40806ES02/03

      0.5 mL/1 mL

      Hieff Trans® mRNA轉(zhuǎn)染試劑

      mRNA

      貼壁和懸浮細(xì)胞

      1.轉(zhuǎn)染效率高
      2.用量少

      40809ES01/03

      0.1 mL/1 mL

       

      Hieff Trans® siRNA/miRNA體外轉(zhuǎn)染試劑

      Hieff Trans® siRNA/miRNA體外轉(zhuǎn)染試劑是一種非脂質(zhì)體PEI陽(yáng)離子、兩性分子轉(zhuǎn)染試劑,為siRNA轉(zhuǎn)染到哺乳動(dòng)物細(xì)胞中而開(kāi)發(fā)。適用于siRNAmiRNA的轉(zhuǎn)染。

      該產(chǎn)品可在廣泛的細(xì)胞系中,實(shí)現(xiàn)1 nMsiRNA超過(guò)90%的表達(dá)效率,避免了脫靶效應(yīng)。適用于多種細(xì)胞轉(zhuǎn)染,包括Hela、MCF-7、HepG2、CHO等貼壁細(xì)胞;以及難以轉(zhuǎn)染的懸浮細(xì)胞系,如K562THP-1細(xì)胞,可達(dá)到80%的沉默效率;同時(shí)還包括一些原代細(xì)胞,原代人成纖維細(xì)胞和原代人肝細(xì)胞等,可達(dá)到80%的沉默效率。

       

      3、DNA/RNA共轉(zhuǎn)染試劑

      產(chǎn)品名稱(chēng)

      核酸類(lèi)型

      細(xì)胞類(lèi)型

      優(yōu)點(diǎn)

      貨號(hào)

      規(guī)格

       Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑

      DNA(小于10 kb)、siRNA

      貼壁細(xì)胞

      1.轉(zhuǎn)染效率高
      2.用量少

      40802ES02/03/08

      0.5 mL/1 mL/5×1 mL

      Hieff Trans®通用型轉(zhuǎn)染試劑

      DNA(小于10 kb/siRNA

      貼壁和懸浮細(xì)胞

      1.毒性低
      2.用量少

      40808ES02/03/08

      0.5 mL/1 mL/5×1 mL

       

      4、工業(yè)生產(chǎn)轉(zhuǎn)染試劑系列產(chǎn)品

      產(chǎn)品名稱(chēng)

      使用場(chǎng)景(包括但不限于)

      細(xì)胞類(lèi)型

      優(yōu)點(diǎn)

      貨號(hào)

      規(guī)格

      線性PEI轉(zhuǎn)染試劑MW40000(速溶型)

      病毒載體、蛋白生產(chǎn)

      CHO系列、HEK-293系列

      1.轉(zhuǎn)染效率高
      2.用量少

      40816ES02/03

      100 mg/1 g

      Hieff Trans® PEI轉(zhuǎn)染試劑

      病毒載體生產(chǎn)(AAV/LV

      HEK-293系列

      1.轉(zhuǎn)染效率高
      2.用量少

      3.GMP級(jí)別

      40820ES04/10/60

      1.5 mL/10 mL/100 mL

       

       

      已驗(yàn)證細(xì)胞系列表

      產(chǎn)品名稱(chēng)

      Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑/40802ES

      已驗(yàn)證部分細(xì)胞系

      293T

      Calu 1

      HEK293

      HO1980

      N2A

      THP-1

      293T(懸浮)

      Chok1

      HEK293T

      HUVEC

      NCI-H1975

      TS cell

      293FT

      COS-7

      Hela

      MCF10A

      NIH-3T3

      U-87

      3T3

      DF-1

      Hep2C

      MCF-7

      Neuro-2a

      Vero

      A549

      H1299

      Hep3B

      MDA-MB-231

      PC-12

      WEHI

      BV-2

      H520

      Hepa1-6

      MDA-MB-231-LM2-4175 

      Raw264.7

      WRL-68

      B50

      HaCaT

      HepG2

      MDCK

      SGC-7901

      多發(fā)性骨髓瘤細(xì)胞

      C2C12

      HCT116

      HK2

      MEF

      T47D

       

      產(chǎn)品名稱(chēng)

      Hieff Trans® siRNA/miRNA體外轉(zhuǎn)染試劑/40806ES

      已驗(yàn)證部分細(xì)胞系

      A549

      HepG2

      MCF-7

      THP-1

       

       

      HEK293T

      CaSki

      RAW264.7

      原代人成纖維細(xì)胞

       

      Hela

      K562

      SiHA

      原代人干細(xì)胞

       

       

      產(chǎn)品名稱(chēng)

      線性PEI轉(zhuǎn)染試劑MW40000(速溶型)/40816ES

      已驗(yàn)證部分細(xì)胞系

      COS-1

      CHO-K1

      HEK-293

      HepG2

      Sf9

       

      COS-7

      Hela

      HEK-293T

      NIH-3T3

       

       

       

      拓展信息

       

      1、脂質(zhì)體與PEI轉(zhuǎn)染試劑的差異,可查看1分鐘解鎖兩代轉(zhuǎn)染試劑的差異
      2、轉(zhuǎn)染試劑常見(jiàn)問(wèn)題,可查看轉(zhuǎn)染效率低?小翊手把手教你做轉(zhuǎn)染實(shí)驗(yàn)太實(shí)用了!轉(zhuǎn)染試劑常見(jiàn)問(wèn)題大全,你想要的答案這里都有

      3Hieff Trans™ Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑針對(duì)不同細(xì)胞中DNA與試劑用量參考,可查看40802參考數(shù)據(jù)

      4、線性PEI轉(zhuǎn)染試劑MW40000(速溶型)進(jìn)一步介紹,可查看轉(zhuǎn)染新寵——PEI MAX (線性PEI MW 40000)更高效的轉(zhuǎn)染試劑

      5、大牛們的高分文章選讀,可查看IF55分!翌圣轉(zhuǎn)染試劑助力高分文章翌圣明星CP--轉(zhuǎn)染試劑與PCR產(chǎn)品又登《Cell》期刊超燃!翌圣轉(zhuǎn)染試劑助力這個(gè)團(tuán)隊(duì)連發(fā)3IF10+文章

       

      產(chǎn)品使用發(fā)表的部分文獻(xiàn)

      向下滑動(dòng)查看

       

      [1] Liu R, Yang J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins. Nat Biotechnol. 2022 Jan 3. (IF:55)華東理工大學(xué)生物工程學(xué)院楊弋團(tuán)隊(duì)

      [2] Luo J, Yang Q, et al. TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022 Mar 17.41.582)西湖大學(xué)陶亮團(tuán)隊(duì)

      [3] Zhou J, Chen P, et al. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. Mol Ther. 2022 Jan 5.(IF:11.454)武漢大學(xué)生命科學(xué)學(xué)院殷雷團(tuán)隊(duì)

      [4] Chen S, Cao X, et al. circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c-Myc Translation. Adv Sci (Weinh). 2022 Jan 24.(IF:16.808)中國(guó)科學(xué)院北京生命科學(xué)研究院趙方慶團(tuán)隊(duì)

      [5] Gu C, Wang Y, et al. AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma. J Exp Clin Cancer Res. 2022 Jan 6.(11.161)南京中醫(yī)藥大學(xué)楊燁顧春艷團(tuán)隊(duì)

      [6] Zhang Y, Yu X, et al. Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication. Clin Transl Med. 2022 Feb.(11.492)南京中醫(yī)藥大學(xué)楊燁顧春艷團(tuán)隊(duì)

      [7] Qin J, Cai Y, et al. Molecular mechanism of agonism and inverse agonism in ghrelin receptor. Nat Commun. 2022 Jan 13.(14.9)四川大學(xué)生物治療國(guó)家重點(diǎn)實(shí)驗(yàn)室邵振華團(tuán)隊(duì)

      [8] Tang X, Deng Z, et al. A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. J Exp Clin Cancer Res. 2022 Mar 8.(11.161)南京中醫(yī)藥大學(xué)楊燁顧春艷團(tuán)隊(duì)

      [9] Xie F, Su P, et al. Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID-19 Therapy. Adv Mater. 2021 Oct 19. (IF:30.849)蘇州大學(xué)生物醫(yī)學(xué)研究院周芳芳團(tuán)隊(duì)和浙江大學(xué)生命科學(xué)研究院張龍團(tuán)隊(duì)

      [10] Liang Y, Lu Q, et al. Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network. Nucleic Acids Res. 2021 Sep 7.(IF:16.9)復(fù)旦大學(xué)生物醫(yī)學(xué)研究院于文強(qiáng)團(tuán)隊(duì)

      [11] Fan Y, Wang J, et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer. 2021 Feb 2.(IF:27.403)南京醫(yī)科大學(xué)附屬逸夫醫(yī)院蘇東明團(tuán)隊(duì)

      [12] Dai L, Dai Y, et al. Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin. Mol Cell. 2021 Jul 1.(IF:17.97)浙江大學(xué)生命科學(xué)研究院黃俊團(tuán)隊(duì)和中科院生物物理所周政團(tuán)隊(duì)

      [13] Zhang K, Wang A, et al. UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. Neuron. 2021 Jun 16.(IF:17.17)中國(guó)科學(xué)院生物與化學(xué)交叉研究中心王文元團(tuán)隊(duì)

      [14] Li T, Chen X, et al. A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. Nat Commun. 2021 Jan 27.(IF:14.92)華東理工大學(xué)生物工程學(xué)院楊弋團(tuán)隊(duì)

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